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Molecular and serological characteristics of hospitalized dengue patients. Serum samples collected at hospital admission were tested by RT‐qPCR for DENV (serotypes 1–4), CHIKV, ZIKV, MAYV, and OROV, and by <t>ELISA</t> for DENV‐specific IgM and IgG. (A) Proportion of patients testing positive for DENV, CHIKV, and DENV/CHIKV coinfection, as well as RT‐qPCR–negative cases. (B) Distribution of DENV serotypes among RT‐qPCR–positive patients, highlighting the predominance of DENV‐1 and the detection of DENV‐2, DENV‐3, and mixed infections (DENV‐1/DENV‐2 and DENV‐1/DENV‐3). No DENV‐4 cases were identified. (C) Serological profiles based on IgM and IgG ELISA results, including single‐ and dual‐antibody positivity and their relationship with RT‐qPCR outcomes, illustrating partial overlap between molecular and serological diagnostic windows.
Panbiotm Dengue Igm Capture Elisa Kit, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Molecular and serological characteristics of hospitalized dengue patients. Serum samples collected at hospital admission were tested by RT‐qPCR for DENV (serotypes 1–4), CHIKV, ZIKV, MAYV, and OROV, and by <t>ELISA</t> for DENV‐specific IgM and IgG. (A) Proportion of patients testing positive for DENV, CHIKV, and DENV/CHIKV coinfection, as well as RT‐qPCR–negative cases. (B) Distribution of DENV serotypes among RT‐qPCR–positive patients, highlighting the predominance of DENV‐1 and the detection of DENV‐2, DENV‐3, and mixed infections (DENV‐1/DENV‐2 and DENV‐1/DENV‐3). No DENV‐4 cases were identified. (C) Serological profiles based on IgM and IgG ELISA results, including single‐ and dual‐antibody positivity and their relationship with RT‐qPCR outcomes, illustrating partial overlap between molecular and serological diagnostic windows.
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igm capture elisa kit  (Abbott Laboratories)
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Molecular and serological characteristics of hospitalized dengue patients. Serum samples collected at hospital admission were tested by RT‐qPCR for DENV (serotypes 1–4), CHIKV, ZIKV, MAYV, and OROV, and by <t>ELISA</t> for DENV‐specific IgM and IgG. (A) Proportion of patients testing positive for DENV, CHIKV, and DENV/CHIKV coinfection, as well as RT‐qPCR–negative cases. (B) Distribution of DENV serotypes among RT‐qPCR–positive patients, highlighting the predominance of DENV‐1 and the detection of DENV‐2, DENV‐3, and mixed infections (DENV‐1/DENV‐2 and DENV‐1/DENV‐3). No DENV‐4 cases were identified. (C) Serological profiles based on IgM and IgG ELISA results, including single‐ and dual‐antibody positivity and their relationship with RT‐qPCR outcomes, illustrating partial overlap between molecular and serological diagnostic windows.
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Molecular and serological characteristics of hospitalized dengue patients. Serum samples collected at hospital admission were tested by RT‐qPCR for DENV (serotypes 1–4), CHIKV, ZIKV, MAYV, and OROV, and by <t>ELISA</t> for DENV‐specific IgM and IgG. (A) Proportion of patients testing positive for DENV, CHIKV, and DENV/CHIKV coinfection, as well as RT‐qPCR–negative cases. (B) Distribution of DENV serotypes among RT‐qPCR–positive patients, highlighting the predominance of DENV‐1 and the detection of DENV‐2, DENV‐3, and mixed infections (DENV‐1/DENV‐2 and DENV‐1/DENV‐3). No DENV‐4 cases were identified. (C) Serological profiles based on IgM and IgG ELISA results, including single‐ and dual‐antibody positivity and their relationship with RT‐qPCR outcomes, illustrating partial overlap between molecular and serological diagnostic windows.
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Molecular and serological characteristics of hospitalized dengue patients. Serum samples collected at hospital admission were tested by RT‐qPCR for DENV (serotypes 1–4), CHIKV, ZIKV, MAYV, and OROV, and by <t>ELISA</t> for DENV‐specific IgM and IgG. (A) Proportion of patients testing positive for DENV, CHIKV, and DENV/CHIKV coinfection, as well as RT‐qPCR–negative cases. (B) Distribution of DENV serotypes among RT‐qPCR–positive patients, highlighting the predominance of DENV‐1 and the detection of DENV‐2, DENV‐3, and mixed infections (DENV‐1/DENV‐2 and DENV‐1/DENV‐3). No DENV‐4 cases were identified. (C) Serological profiles based on IgM and IgG ELISA results, including single‐ and dual‐antibody positivity and their relationship with RT‐qPCR outcomes, illustrating partial overlap between molecular and serological diagnostic windows.
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Molecular and serological characteristics of hospitalized dengue patients. Serum samples collected at hospital admission were tested by RT‐qPCR for DENV (serotypes 1–4), CHIKV, ZIKV, MAYV, and OROV, and by <t>ELISA</t> for DENV‐specific IgM and IgG. (A) Proportion of patients testing positive for DENV, CHIKV, and DENV/CHIKV coinfection, as well as RT‐qPCR–negative cases. (B) Distribution of DENV serotypes among RT‐qPCR–positive patients, highlighting the predominance of DENV‐1 and the detection of DENV‐2, DENV‐3, and mixed infections (DENV‐1/DENV‐2 and DENV‐1/DENV‐3). No DENV‐4 cases were identified. (C) Serological profiles based on IgM and IgG ELISA results, including single‐ and dual‐antibody positivity and their relationship with RT‐qPCR outcomes, illustrating partial overlap between molecular and serological diagnostic windows.
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Molecular and serological characteristics of hospitalized dengue patients. Serum samples collected at hospital admission were tested by RT‐qPCR for DENV (serotypes 1–4), CHIKV, ZIKV, MAYV, and OROV, and by <t>ELISA</t> for DENV‐specific IgM and IgG. (A) Proportion of patients testing positive for DENV, CHIKV, and DENV/CHIKV coinfection, as well as RT‐qPCR–negative cases. (B) Distribution of DENV serotypes among RT‐qPCR–positive patients, highlighting the predominance of DENV‐1 and the detection of DENV‐2, DENV‐3, and mixed infections (DENV‐1/DENV‐2 and DENV‐1/DENV‐3). No DENV‐4 cases were identified. (C) Serological profiles based on IgM and IgG ELISA results, including single‐ and dual‐antibody positivity and their relationship with RT‐qPCR outcomes, illustrating partial overlap between molecular and serological diagnostic windows.
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Molecular and serological characteristics of hospitalized dengue patients. Serum samples collected at hospital admission were tested by RT‐qPCR for DENV (serotypes 1–4), CHIKV, ZIKV, MAYV, and OROV, and by <t>ELISA</t> for DENV‐specific IgM and IgG. (A) Proportion of patients testing positive for DENV, CHIKV, and DENV/CHIKV coinfection, as well as RT‐qPCR–negative cases. (B) Distribution of DENV serotypes among RT‐qPCR–positive patients, highlighting the predominance of DENV‐1 and the detection of DENV‐2, DENV‐3, and mixed infections (DENV‐1/DENV‐2 and DENV‐1/DENV‐3). No DENV‐4 cases were identified. (C) Serological profiles based on IgM and IgG ELISA results, including single‐ and dual‐antibody positivity and their relationship with RT‐qPCR outcomes, illustrating partial overlap between molecular and serological diagnostic windows.
Dengue Igm Capture Elisa Kit, supplied by InBios International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Molecular and serological characteristics of hospitalized dengue patients. Serum samples collected at hospital admission were tested by RT‐qPCR for DENV (serotypes 1–4), CHIKV, ZIKV, MAYV, and OROV, and by <t>ELISA</t> for DENV‐specific IgM and IgG. (A) Proportion of patients testing positive for DENV, CHIKV, and DENV/CHIKV coinfection, as well as RT‐qPCR–negative cases. (B) Distribution of DENV serotypes among RT‐qPCR–positive patients, highlighting the predominance of DENV‐1 and the detection of DENV‐2, DENV‐3, and mixed infections (DENV‐1/DENV‐2 and DENV‐1/DENV‐3). No DENV‐4 cases were identified. (C) Serological profiles based on IgM and IgG ELISA results, including single‐ and dual‐antibody positivity and their relationship with RT‐qPCR outcomes, illustrating partial overlap between molecular and serological diagnostic windows.
Dengue Igg Capture Elisa Kit, supplied by InBios International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Molecular and serological characteristics of hospitalized dengue patients. Serum samples collected at hospital admission were tested by RT‐qPCR for DENV (serotypes 1–4), CHIKV, ZIKV, MAYV, and OROV, and by ELISA for DENV‐specific IgM and IgG. (A) Proportion of patients testing positive for DENV, CHIKV, and DENV/CHIKV coinfection, as well as RT‐qPCR–negative cases. (B) Distribution of DENV serotypes among RT‐qPCR–positive patients, highlighting the predominance of DENV‐1 and the detection of DENV‐2, DENV‐3, and mixed infections (DENV‐1/DENV‐2 and DENV‐1/DENV‐3). No DENV‐4 cases were identified. (C) Serological profiles based on IgM and IgG ELISA results, including single‐ and dual‐antibody positivity and their relationship with RT‐qPCR outcomes, illustrating partial overlap between molecular and serological diagnostic windows.

Journal: Journal of Medical Virology

Article Title: Clinical, Serological, and Molecular Profile of Dengue Patients With Warning Signs During the 2024 Outbreak in Belo Horizonte, Brazil

doi: 10.1002/jmv.70805

Figure Lengend Snippet: Molecular and serological characteristics of hospitalized dengue patients. Serum samples collected at hospital admission were tested by RT‐qPCR for DENV (serotypes 1–4), CHIKV, ZIKV, MAYV, and OROV, and by ELISA for DENV‐specific IgM and IgG. (A) Proportion of patients testing positive for DENV, CHIKV, and DENV/CHIKV coinfection, as well as RT‐qPCR–negative cases. (B) Distribution of DENV serotypes among RT‐qPCR–positive patients, highlighting the predominance of DENV‐1 and the detection of DENV‐2, DENV‐3, and mixed infections (DENV‐1/DENV‐2 and DENV‐1/DENV‐3). No DENV‐4 cases were identified. (C) Serological profiles based on IgM and IgG ELISA results, including single‐ and dual‐antibody positivity and their relationship with RT‐qPCR outcomes, illustrating partial overlap between molecular and serological diagnostic windows.

Article Snippet: For serological evaluation, ELISA was performed on patient sera using the PanbioTM Dengue IgM Capture ELISA kit (Abbott, EUA) for IgM detection and the Euroimmun Dengue Virus IgG ELISA kit (Euroimmun, Germany) for IgG detection.

Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Diagnostic Assay